No ke aha ka hāpana ua paa Coombs?

Coombs - he mau 'keʻena hoʻokolohua ho'āʻo e leie aku e hooholo i nā nā mea'aʻaʻa i loko o ka wai koko a me kaʻulaʻula koko aeea ili honua. Kēia kaʻina hana e leie aku e diagnose'ōnaehana paleʻea hemolytic anaemia, a me ke keiki, e like me ke kuhikuhi i hemolytic transfusion reactions. Coombs ua 'Imi hoʻohana' ia i loko o forensic 'epekema a me ka aaiaoe? Noiʻi, e hooholo i ka erythrocyte antigens. Hoʻokō 'ana i ka rula a pau o ia ka Ka Ikepiliʻo ia ka mea hiki ke kiʻi i ka loa ike loea hopena.

Ke kumu o antiglobulin hoʻokolohua

Direct Coombs hōʻike hiki huai ae Anati-erythrocytic nā mea'aʻaʻa, a i paa ma ka erythrocytes. Maikaʻi naoh ka wā i like study kuhikuhi mai la lakou i ka ulu ana o autoimmune hemolytic anaemia. It E e kaulana i ka io result 'aʻole e hookaawale ia i ke alo o autoimmune maʻi, mai nā mea'aʻaʻa pinepine i loaʻa i loko o noa palapala, a laila i ole pili me erythrocytes. Ma na hihia ia mea kupono e lawe mai i kaʻauhau'ēʻaʻe Coombs hōʻike, a e hooholo i ka hana nona iho konohiki ma ka lāʻau lapaʻau.

Pehea o ke Ka Ikepili?

W Pūʻolo o venous koko o ka hoʻomanawanui ua lawe mai ma luna o ka waiwaiʻole'ōpū i loko o ke kakahiaka, hoʻowahāwahā i ka mea i kekahiʻiʻo aʻe e ho ololi i ka hopena hope loa o kēia hōʻike, ka mea, ua i hoikeia. Store mea aeia mai ia ma ka 2 a hiki i 8 ° C ole hou aku i na la ehiku. No nā hoailona o keia ao, ua like kūlike loa i hiki, ka mea a pau iloko o ke koko o ka, elua no hora mua ia e haawi ana i ke keʻena hoʻokolohua. Maikaʻi loa Coombs hāpana e hōʻike i ka io hopena, e hoike ana i ka wa e kaawale aku o hemolytic loli i loko o ke kino.

Deciphering Ka lōʻihi o ke

Coombs Heʻano laborious noiʻi hana, i pono pono a me ka pololei. I ka hoʻohana 'ia ka hōʻike i ke pilikia kekahi pilikia e i pili i ka mea pololei ole ano o ka hope loa hualoaʻa aie i ka nawaliwali hoike ana o ka maikaʻi reactions. It E e hoʻomaopopo i ka unreliability Ka Ikepili - o maikaʻi Coombs - i e kaʻilihune o inefficient holoi erythrocytes hui 'ana me momoma
ili a me ka neutralization eiiiiiaiou antiglobulin reagents lāʻau lapaʻau. Kekahi keakea o keia iaoia o ka instability o ka hoʻopaʻa 'ana i ia mea waihoia i loaʻa kekahi mau hiʻona.

Ke kumu o ka wahahee-io result hiki e excessive lulu ka wehe ana o erythrocytes ma resuspension. Erroneous hualoaʻa i kekahi e ku pono i ke alo o anticomplement lāʻau lapaʻau kino impurities i i adsorbed ma luna o ka ili i ka incubation o ka hoao ana erythrocytes, ma laila e pili ana i ka helehelena o ka maikaʻi hopena. Inā 'oe pono holoi ho'āʻo Eia kekahi laʻana, a kanaka hoʻoponopono i ka naoh keia, mau keakea hiki wale ke eliminated, a e hoomahuahua i ka manawa o ka noho i ka loa ike loea nā hoailona o Coombs hōʻike.