Keʻano papa hana o ka hana o nā'ūhū

Nā'ūhū (nā'ūhū) - he kiʻekiʻe hakakino kaumaha ke aloha i huiʻia o ka kumuʻiʻo ano, i hana i loko o ka meaola i kekahi kūlana o liluwelo catalysts.

Keʻano papa hana o ka hana o nā'ūhū

Elucidation o na maomeka hp'pnphp ka catalytic hana o nā'ūhū, o kekahi o na kumu pilikia a me ka ʻikena pilikia ole wale enzymology, akā, laekahi biochemistry a me hakakino kālaimeaola.

Loa ma mua o nā nā'ūhū lilo loaʻa maemae, a ua elucidated lākou ano, ua lilo i hoomaopopoia iʻano nui no ka enzymatic kaʻina ka mea, i ka hui ana o ka'ūhū i ka kūpapakū. Ho'āʻo 'o ia e' imi i ka luna 'huina lakou o ka'ūhū me ka kūpapakū no ka manawa lōʻihi i i alakai i ka pomaikai, e like me keia luna' ka labile, e loa hikiwawe loa decomposes. E ho ohana i spectroscopy hana ua i ka mea hiki ke kuhikuhi i ka'ūhū-kūpapakū pono a pau o ia catalase, peroxidase, waiʻona dehydrogenase, flavinzavisimyh nā'ūhū.

I ka papa hana o ka X-Ray Ka Ikepili ua 'ae' e kiʻi i ka hailona o ka nui 'ike ma luna o ka' ole a me ka catalytic maomeka o ka hana o nā'ūhū. Kēia papa hana i hoʻohana 'e hookupaa nui ae i ka pili kūpapakū analogues me nā'ūhū lysozyme a me ka chymotrypsin.

Kekahi pololei hoike no ke ola ana o ka'ūhū-kūpapakū eiiieaenu e hoʻokele e kiʻi i kahi i kekahi o ka catalytic kalapona kuʻina o ka'ūhū ua paa ia i ka kūpapakū ma ka covalent hoopaa. Lakou i mea hoike o ka naoh o hydrolysis o ka n-nitrophenyl'ākeka catalyzed ma chymotrypsin. I ka wa i hui pūʻia ai me ka'ūhū chymotrypsin Etera ua acetylated ma ka hydroxyl pae o ka kū'ē serine koena. Kēia kahua He kokololio, akā, atsetilhimotripsina hydrolysis me ke kahua o ka'ākeka a me ka noa chymotrypsin mea nui ke kua'āina. No ia mea, atsetilhimotripsin accumulates, i ua hikiwawe akomi i loko o ke alo o ka n-nitrophenylacetate.

Ke alo aku o ka'ūhū kūpapakū i loko o ka haku mele 'ana e hiki "hoopahele ae la i" ma ka hoolilo eu lolelua ka luna' i loko o ka AEeOEXAaI ano, no ka laʻana, ma ka lapaau ana me ka'ūhū-kūpapakū luna 'o ka paʻakai borohydride me ka ikaika hoemi kanawai. Oia ka luna 'i loko o ka palapala ana i ka pilina paʻa covalent derivative i' akomi i loko o ka'ūhū aldolase. Ua Ua loaʻa i ke kūpapakū hana me ka lātoma e-'amino pae o ka lysine.

Ke kūpapakū reacts me ka'ūhū i loko o kekahi ua loa i ua kapaia kaʻeleu waena o ka'ūhū a me kaʻeleu wahi.

Ma lalo o kaʻeleu iwaena konu, a me Kora naʻEsau, maopopo i ka hapa'ūhū kumuʻiʻo lātoma a ua pili i ka kūpapakū (a me cofactors) a me ke kumu o ka enzymatic waiwai o ka lātoma. Kaʻeleu paena e hoʻoholo i ka specificity a me catalytic aayoaeuiinoe o ka'ūhū, a ina e i kekahiʻano o ka pilikia 'ole hakuloli no ke kokoke loaʻana a me ka launa pū' ana me ka kūpapakū lātoma, a me kona mau māhele pili 'ole i loko o ka naoh.

Mawaena o ka loiloi pūʻulu i ike okoa ia i komo i loko o ka "catalytically hana" hapa o ka'ūhū, a me ka ho okumu i hapa ka hoʻolako 'ana i kēiaʻo Solomona (o ka paa ana o kūpapakū i ka'ūhū) - ka ai-kapaia kui, a "heleuma" (a adsorption o ka'ūhūʻeleu paena).

Keʻano papa hana o ka hana o nā'ūhū wehewehe i ka kumumanaʻo o ka Michaelis-Menten helu kaulike. E like me ia i kēia kumumanaʻo, ke kaʻina i wahi i ka eha kahua o ka hoʻonaʻauao.

Keʻano papa hana o ka hana o nā'ūhū: I pae

Ma waena o ka kūpapakū (C) a me ka'ūhū (E) i ka pilina aicieeaao - hana eu'ūhū-kūpapakū eiiieaen, kahi o nā eiiiiiaiou i interconnected ma ka covalent, ionic, ka wai, a me ka paa.

Keʻano papa hana o ka hana o nā'ūhū: IІ ke kahua

Ke kūpapakū ua olohelohe ia lakou i ka mea i pili'ūhū ua ho'ā 'ia paha, a i loaʻa no ka lakou mau reactions o ka eu catalysis.

Keʻano papa hana o ka hana o nā'ūhū: IІI ke kahua

Eu hana catalysis. Kēia kumumanaʻo ua i kākoʻo 'ia ma ka mana haʻawina.

Eia hoi, IV kahua ua wehewehe aku ma ke kala ana o ka'ūhū nā lātoma E a me F. ke kaʻina o nā loli 'o nā huahana hiki ke hōʻike me ka naoh: E + S - eu eu - * - E + P.

Ke specificity o'ūhū 'ana

Kēlā me kēia'ūhū hana ma luna o kekahi mau 'kūpapakū a pae o ka waiwai, a e like me ka' ole. Specificity o enzymatic ha awina ma muli o ia aʻo Kīwala'ō o kaʻeleu paena hoʻonohonohoʻia a me ka kūpapakū. Iloko o ka AaOIeIOUIeXAOAaIN o'ūhū-kūpapakū hana luna '.